FOXP3 expression in peripheral blood and synovial cells of patients with juvenile idiopathic arthritis: relationship with IL-17 at cytokine and molecular level
نویسندگان
چکیده
Materials and methods We have enrolled 58 JIA patients (polyarticular and oligoarticular disease) and 69 healthy controls. We examined CD4+CD25+FOXP3+ percentage (flow cytometry), FOXP3 and RORγt mRNA (RT-PCR) in peripheral blood mononuclear cells (PBMCs), and in synovial fluid mononuclear cells (SFMCs). FOXP3 median fluorescence intensity (MFI) of CD4+ T cells was also determined. Interleukin-17 levels were measured (ELISA) in stimulated PBMCs supernatants in 22 patients.
منابع مشابه
Interleukin-17–producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers
OBJECTIVE To identify interleukin-17 (IL-17)-producing T cells from patients with juvenile idiopathic arthritis (JIA), and investigate their cytokine production, migratory capacity, and relationship to Treg cells at sites of inflammation, as well as to test the hypothesis that IL-17+ T cell numbers correlate with clinical phenotype in childhood arthritis. METHODS Flow cytometry was used to an...
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